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KMID : 0379520060220020075
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한국독성학회지
2006 Volume.22 No. 2 p.75 ~ p.85
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Assessment of DNA Damage using an Alkaline Single Cell Gel Electrophoresis (SCGE) Comet Assay and Toxic Effects in Chickens by T-2 Toxin Treatment
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Hah Dae-Sik
Heo Jung-Ho
Lee Kuk-Cheon
Cho Myung-Heui
Kim Kuk-Hun
Kim Chung-Hui
Lue Jae-Du
Lee Seung-Hwan
Kim Gon-Sup
Kim Eui-Kyung
Kim Jong-Shu
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Abstract
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This study was designed to evaluate the possible DNA damaging effects of T-2 toxin using an alkaline single cell gel electrophoresis (SCGE) comet assay and also to investigate toxic effects in chickens. A total of 20 chickens were used in these experiments. Graded concentrations of dietary T-2 toxin (0, 4, 8, and 16{mu}g/g of diet) were given to groups of 5 broiler chickens. In comet assay, The DNA damage was analysed by the tail extent moment (TEM) and tail length (TL), which were used as markers of DNA strand breaks in SCGE. A significant dose-dependent increase in the extent of DNA migration as well as in the percentage of cells with tails was observed after treatment with T-2 toxin (P<0.05). Treatment with the low T-2 toxin (4{mu}/g of diet) induced a relatively low level of DNA damage in comparison with the high T-2 toxin (16{mu}/g of diet) group. The growth rate was significantly reduced by concentrations of 8, and 16{mu}/g of diet (P < 0.05). The feed conversion ratio were significantly affected by any concentrations (P < 0.05). The relative weight of the spleen, and lung was decreased by the growth inhibitory concentrations. The bursa of Fabricius, thymus, and kid- ney were decreased in relative weight by concentrations of 16{mu}/g of diet. The relative weight of the liver and heart were unaffected. The hemoglobin (Hb), hematocrit (HCT), and mean corpuscular hemoglobin (MCH) were decreased at concentration of 16{mu}/g of diet. As compared with control chickens, there was no marked change in serum components except uric acid in T-2 treated chickens. All lymphoid tissues retained atrophic and lymphoid cell depletion throughout the three weeks trial.
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KEYWORD
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Alkaline single cell gel electrophoresis (SCGE) comet assay, T-2 toxin, DNA damage, Toxicity
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